6.1.1.14 - 6.1.1.14 2 enzyme forms: E1 and E2 6.1.1.14 glutathione Sepharose column chromatography 6.1.1.14 glutathione-Sepharose column chromatography, and gel filtration 6.1.1.14 Ni-affinity column chromatography 6.1.1.14 Ni-NTA column chromatography 6.1.1.14 recombinant C-terminally His6-tagged cytosolic enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 6.1.1.14 recombinant enzyme from Escherichia coli, 10fold 6.1.1.14 recombinant His-atgged wild-type enzyme and P552F mutant from Escherichia coli 6.1.1.14 recombinant His-tagged isozymes GlyRS1 and 2 from Escherichia coli 6.1.1.14 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 6.1.1.14 recombinant isozyme GlyRS1 from Escherichia coli 6.1.1.14 Superdex 200 gel filtration