5.4.2.12 - 5.4.2.12 amylose column chromatography 5.4.2.12 HiTrap nickel chelating column chromatography and HiTrap Q column chromatography 5.4.2.12 metal affinity Talon column chromatography 5.4.2.12 Ni-NTA agarose column chromatography 5.4.2.12 Ni-NTA bead chromatography 5.4.2.12 Ni-NTA column chromatography 5.4.2.12 Ni-NTA Sepharose column chromatography and Superdex 200 gel filtration 5.4.2.12 partially purified 5.4.2.12 partialy purified 5.4.2.12 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography and gel filtration 5.4.2.12 recombinant His6-tagged enzyme from Escherichia coli strain C2566/T7 by nicke affinity chromatography, dialysis and anion exchange chromatography 5.4.2.12 recombinant His6-tagged enzyme from Escherichia coli strain C2566/T7 by nickel affinity chromatography, dialysis and anion exchange chromatography 5.4.2.12 recombinant iPGAM 5.4.2.12 recombinant iPGM 5.4.2.12 recombinant phosphoglycerate mutase 5.4.2.12 recombinant protein 5.4.2.12 recombinant protein expressed in Escherichia coli