4.1.2.38 - 4.1.2.38 by Ni2+-NTA affinity column, to purity 4.1.2.38 immobilized metal ion chromatography, after which the enzyme shows only 35-45% of its activity, however, activity is completely regained after incubation in the presence of Mg2+ and thiamine diphosphate 4.1.2.38 Ni-Sepharose column chromatography 4.1.2.38 nickel-affinity HiTrap agarose column chromatography and Sephadex S-200 gel filtration 4.1.2.38 strain biovar I, partial 4.1.2.38 using Ni2+-affinity chromatography