3.6.5.6 - 644199, 644200, 644202, 644203, 644204, 644206, 644207, 644208, 644209, 644210, 644211 3.6.5.6 bovine brain tubulin is purified by two cycles of polymerization and depolymerization 685083 3.6.5.6 goat brain microtubule protein is isolated by two cycles of polymerization and depolymerization, MAP-free tubulin is purified from the microtubule protein by phosphocellulose chromatography 686645 3.6.5.6 MAP-free tubulin is purified from the microtubule protein of goat brain by phosphocellulose chromatography 686670 3.6.5.6 microtubules are prepared from freshly isolated lens epithelial fractions from mice 686631 3.6.5.6 ovine brain tubulin is prepared from freshly-killed animals using phosphocellulose chromatography 685385 3.6.5.6 purified to ca. 35% 644205 3.6.5.6 recombinant His-tagged beta-tubulin mutant T238A from Saccharomyces cerevisiae by nickel affinity chromatography 733717 3.6.5.6 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by dialysis and nickel affinity chromatography, tag cleavage by thrombin, and dialysis 733983 3.6.5.6 using a glutathione-Sepharose affinity column 687534 3.6.5.6 using a Ni-nitrilotriacetic acid resin column 687396