3.2.1.141 - 26155, 26156, 721859, 726343, 726451 3.2.1.141 by Ni2+-NTA-agarose affinity chromatography 681347 3.2.1.141 Ni2+-NTA-agarose column chromatography, gel filtration 715891 3.2.1.141 partial 26157 3.2.1.141 Q-Sepharose anion exchange column chromatography 692727 3.2.1.141 recombinant 669851 3.2.1.141 recombinant His6-tagged enzyme mutant from Escherichia coli by nickel affinity chromatography, followed by anion exchange chromatography, and multiple dialysis steps 753523 3.2.1.141 recombinant wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography and gel filtration 692726 3.2.1.141 wild-type enzyme purified by heat treatment, nucleic acid precipitation, and ion-exchange chromatography, 12fold. His-tagged MTHase partially purified from the cell-free extract directly by metal chelating chromatography, 14.7fold 680283