3.1.3.75 - 679380, 680993, 696420 3.1.3.75 from solubilized inclusion bodies, ion-exchange chromatography (Q-Sepharose), dialysis, gel filtration 706604 3.1.3.75 immobilized metal ion affinity chromatography (Ni2+) 702538 3.1.3.75 Ni2+-Sepharose activated column 714360 3.1.3.75 recombinant enzyme from Escherichia coli by chitin affinity chromatography 664697 3.1.3.75 recombinant His-tagged wild-type and mutant fragments M19-C267 from Escherichia coli by nickel affinity chromatography to homogeneity 664211 3.1.3.75 recombinant N-terminally His6-tagged PchP from Escherichia coli by nickel affinity chromatography 713654 3.1.3.75 recombinant PchP from Escherichia coli, as an N-terminal fusion to intein or a histidine tag, in the folded state 714872 3.1.3.75 recombinant PHOSPHO1 654549, 655132