2.8.3.5	-	
2.8.3.5	4 isozymes, separable by isoelectric focusing	
2.8.3.5	as described in literature	
2.8.3.5	BioSep-SEC-S 3000 gel filtration	
2.8.3.5	gel filtration	
2.8.3.5	HisTrap column chromatography, and Superdex 200 gel filtration	
2.8.3.5	homogeneity	
2.8.3.5	N-terminal and C-terminal domain, a mixture of the domains is only active when domains are isolated in the presence of 2-mercaptoethanol	
2.8.3.5	native enzyme from mitochondria by chromatofocusing and gel filtration to over 75% purity	
2.8.3.5	native enzyme from mitochondria by chromatofocusing and gel filtration to over 85% purity	
2.8.3.5	Ni-NTA agarose column chromatography and Ultrogel AcA 44 column chromatography	
2.8.3.5	Ni-NTA column chromatography and Superdex 200 gel filtration	
2.8.3.5	partial	
2.8.3.5	recombinant ScoA and ScoB by metal affinity chromatography and gel filtration	
2.8.3.5	recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) to homogeneity	
2.8.3.5	two subunits A and B, both subunits are necessary for enzyme activity