2.7.7.B22 - 2.7.7.B22 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography 2.7.7.B22 recombinant N-terminally thioredoxinA-6xHis-tagged wild-type and mutant enzymes from Escherichia coli strain RosettaII(DE3) by nickel affinity chromatography 2.7.7.B22 recombinant soluble His6-tagged enzyme from Escherichia coli strain Rossetta 1 (DE3) by nickel affinity chromatography, identification of the recombinant enzyme by MALDI-TOF-TOF tandem MS analysis 2.7.7.B22 recombinant TnsC1-85 MBP-fusion protein from Escherichia coli strain BL21Star(DE3) pET101/DTOPO-TnsD1–309His, co-purification of the TnsC and TnsD fragments by nickel affinity chromatography, TnsC1-85 interacts directly with TnsD1-309 2.7.7.B22 soluble His-tagged PAI subdomain of SB transposase from Escherichia coli strain BL21-A1 by nickel affinity chromatography