2.7.7.62 - 2.7.7.62 cell-free extracts are prepared 2.7.7.62 in two steps by hydrophobic interaction and ion-exchange chromatography 2.7.7.62 single-step method based on hydrophobic interaction chromatography 2.7.7.62 tandem hydrophobic interaction chromatography on two HiTrap Phenyl FastFlow columns, dialysation, and dye ligand chromatography on HiTrap Blue HP column (elution with 2 M NaCl)