2.7.1.45	-	
2.7.1.45	Butyl Toyopearl column chromatography and Superdex 200 column gel filtration	
2.7.1.45	DEAE Sepharose column chromatography, Q Sepharose column chromatography, Affi-gel blue column chromatography, Sephadex G-75 gel filtration, and Mono Q HR 5/5 column chromatography	
2.7.1.45	recombinant enzyme from Escherichia coli, by heat precipitation and gel filtration	
2.7.1.45	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	
2.7.1.45	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and desalting gel filtration	
2.7.1.45	recombinant protein