2.7.1.151 ammonium sulfate, Con A-Sepharose, DEAE-Toyopearl, 5'AMP-Sepharose 2.7.1.151 ammonuium sulfate, DEAE-cellulose 2.7.1.151 native enzyme partially by nuclear fraction preparation 2.7.1.151 recombinant enzyme 2.7.1.151 recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by ion exchange and phosphocellulose resin chromatography 2.7.1.151 recombinant enzyme from strain expression in strain 12S16cpep4D by nickel affinity chromatography 2.7.1.151 recombinant GST-fusion isozymes from Escherichia coli by glutathione affinity chromatography 2.7.1.151 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity and heparin affinity chromatography, followed by tag cleavage through TEV protease, another step of heparin affinity chromatography, and gel filtration 2.7.1.151 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography 2.7.1.151 recombinant wild-type enzyme and deletion mutant from Escherichia coli by ion exchange and phosphocellulose resin chromatography