2.5.1.20	-	
2.5.1.20	ammonium sulfate, DE52, Sephacryl S-200, hexyl-Sepharose, Mono Q	
2.5.1.20	ammonium sulfate, Sephadex G-25, DEAE-cellulose, partial purification	
2.5.1.20	centrifugation to separate small rubber particles from the lower layer, dilution with 0.01 M phosphate buffer, pH 7.0 to 30% dry rubber content, washed particles are produced by washing with 0.25 M Tris-HCl, pH 8.0, with 1% Tween-20 and 5 mM 2-mercaptoethanol, applied to a Ultrogel AcA-44 column, centrifuged and diluted to 15% dry rubber content, highly purified small particles are produced by further centrifugation with 0.5% Triton X-100, exchange by buffer and centrifugation, cream phase diluted to 15% dry rubber content	
2.5.1.20	Ni-NTA resin column chromatography	
2.5.1.20	Parthenium argentatum makes rubber in parenchyma cells, which must be homogenized to release the rubber particles, the method for purification of enzymatically active rubber particles from the plant requires homogenization that also releases copious amounts of proteases and other degradative enzymes, with a resultant decrease in enzyme stability and half-life, overview	
2.5.1.20	partial	
2.5.1.20	partially	
2.5.1.20	pH 5.4, ammonium sulfate, Sephadex G-100, DEAE-Sephadex, partial purification	
2.5.1.20	purification of rubber particles containing the enzyme by harvesting latex from petioles of roots, separating latex fractions by centrifugation with extraction buffer (100 mM Tris-HCl, pH 7.8, 350 mM sorbitol, 10 mM NaCl, 5 mM MgCl2, 5 mM DTT), rubber phase is further centrifuged, washed with buffer and used for enzyme assays