2.4.1.11 Bio-Scale Mini Profinity IMAC 2.4.1.11 Hi Trap column chromatography and Sepharose 6 column chromatography 2.4.1.11 His6-tagged recombinant proteins 2.4.1.11 HisTrap column chromatography and Superdex 75 gel filtration 2.4.1.11 recombinant His-tagged wild-type and mutant Gsy2 proteins from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography 2.4.1.11 the COOH-terminal fragment of glycogenin can be used as an effective high affinity reagent for the purification of glycogen synthase from skeletal muscle and liver