2.1.1.224	C-terminally His6-tagged wild-type enzyme and mutant enzymes C112A, C116A and C119A	
2.1.1.224	recombinant His6-tagged Cfr from Escherichia coli strain BL21Star by nickel affinity chromatography and gel filtration	
2.1.1.224	recombinant tagged enzyme Cfr(E) from Escherichia coli strain Rosetta(DE3)pLysS by metal chelating chromatography