1.5.1.33 - 1.5.1.33 HisTrap nickel-chelating column chromatography 1.5.1.33 native and recombinant enzyme 1.5.1.33 recombinant enzyme 1.5.1.33 recombinant enzyme from Escherichia coli 1.5.1.33 recombinant His-tagged enzyme from Escherichia coli 1.5.1.33 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)by nickel affinity chromatography 1.5.1.33 recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, and gel filtration 1.5.1.33 T7 Tag antibody agarose column chromatography 1.5.1.33 using Ni-NTA chromatography