1.3.1.45	-	
1.3.1.45	as six-His tagged OsIRL protein, the generated recombinant OsIRL protein is used for antibody preparation	
1.3.1.45	Ni2+-NTA affinity chromatography and gel filtration	
1.3.1.45	partial	
1.3.1.45	partially, subcellular fractions of cultured cells	
1.3.1.45	recombinant His-tagged enzyme from cell free extract of Escherichia coli strain BL21(DE3)	
1.3.1.45	using Ni-NTA agarose affinity chromatography