1.2.1.22	-	
1.2.1.22	anion-exchange chromatography	
1.2.1.22	by gel filtration	
1.2.1.22	mutant F180T purified to homogeneity (more than 95% pure), on Ni2+ resin	
1.2.1.22	Ni-affinity chromatography	
1.2.1.22	nickel-chelating affinity column	
1.2.1.22	partial	
1.2.1.22	recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange chromatography to near homogeneity	
1.2.1.22	three chromatography steps