1.2.1.16 - 288041, 288046 1.2.1.16 ammonium sulfate fractionation and DEAE-cellulose 288042 1.2.1.16 ammonium sulfate fractionation, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography 678422 1.2.1.16 by ammonium sulfate precipitation and anion exchange chromatography and gel filtration 698627 1.2.1.16 chromatography on Celite 535 288050 1.2.1.16 chromatography on Sephacryl S-300, DEAE-Sephacel and Sepharose 4B 288038 1.2.1.16 column chromatography on DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex 288044 1.2.1.16 DEAE-cellulose chromatography 287170 1.2.1.16 His-tagged GabD dehydrogenase purified from cell extracts with HiTrap affinity columns 692836 1.2.1.16 Ni-NTA resin column chromatography 724115 1.2.1.16 Ni-NTA resin column chromatography and Superdex 75 gel filtration 726463 1.2.1.16 Ni2+-agarose chromatography 685387 1.2.1.16 polyanionic resin Polyanion S1-HR5/5 288037 1.2.1.16 Purification of his-tagged proteins by using a standard Ni2+-agarose chromatography protocol. 685387 1.2.1.16 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 763626 1.2.1.16 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis 762770 1.2.1.16 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration 743077 1.2.1.16 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 743290 1.2.1.16 UNO Q-12 column chromatography 722211 1.2.1.16 UNO Q-12 column chromatography and Superdex 200 gel filtration 722211