1.14.15.18 - 1.14.15.18 full-length and truncated enzyme forms from liver mitochondria 1.14.15.18 partially 1.14.15.18 purification of 1alpha-hydroxylating cytochrome P-450 1.14.15.18 purification of cytochrome P-450D1alpha 1.14.15.18 purification of ferredoxin component 1.14.15.18 recombinant His-tagged CYP27B1 from Escherichia coli strain JM109 by nickel affinity chromatoghraphy, followed by affinity chromatography on a bovine adrenodoxin-coupled resin with elution by 1.0% CHAPS 1.14.15.18 recombinant wild-type Vdh and mutant Vdh-K1 from Escherichia coli 1.14.15.18 vitamin D3 25- and 1alpha-hydroxylase