1.14.14.22 native enzyme 102fold to homogeneity by two different steps of anion exchange chromatography, followed by hydrophobic interaction chromatography, and gel filtration 1.14.14.22 native enzyme 102fold to homogeneity from strain D-1 by ammonium sulfate fractionation, two different steps of anion exchange chromatography, hydrophobic interaction chromatography, gel filtration 1.14.14.22 native enzyme 47.6fold by anion exchange and hydrophobic interaction chromatography, followed by another different step of anion exchange chromatography and hydroxyapatite chromatography 1.14.14.22 native enzyme 82.6fold from strain WU-S2B by three different steps of anion exchange chromatography and one step of hydrophobic interaction chromatography, gel filtration, and another step of anion exchange chromatography, recombinant enzyme from Escherichia coli 17.0fold to homogeneity by anion exchange and hydrophobic interaction chromatography, and again anion exchange chromatography 1.14.14.22 native enzyme from strain IGTS8 by anion exchange chromatography and gel filtration 1.14.14.22 native enzyme to homogeneity by anion exchange and hydrophobic interaction chromatography, and gel filtration 1.14.14.22 recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinty chromatography