1.12.1.4	-	
1.12.1.4	DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration	
1.12.1.4	nickel affinity column chromatography and Superdex 200 gel filtration	
1.12.1.4	nickel affinity column chromatography, and Superose 12 gel filtration	
1.12.1.4	streptactin column chromatography	
1.12.1.4	under strictly anoxic conditions, the enzyme is inactivated in the presence of even trace amounts of O2