1.1.2.8 - 1.1.2.8 C-terminally His-tagged PQQ-ADHs are expressed in Escherichia coli BL21(DE3) 1.1.2.8 native enzyme from membranes by two steps of anion exchange chormatography, hydroxyapatite chromatography, and gel filtration 1.1.2.8 native enzyme from strain IFO3191 by ultracentrifugation and anion exchange chromatography 1.1.2.8 native enzyme from strain MSU10 by ultracentrifugation and anion exchange chromatography 1.1.2.8 native enzyme from strain SKU1108 by ultracentrifugation and anion exchange chromatography 1.1.2.8 recombinant His6-tagged enzyme from Escherichia coli strain TOP10 by ultracentrifugation, nickel affinity chromatography, ultrafiltration, and desalting gel filtration