1.1.2.7	-	
1.1.2.7	Ca2+-containing and Ca2+-free enzymes from cell-free extracts by ion exchange and hydrophobic interaction chromatography	
1.1.2.7	native active alpha2beta2 MDH complex by ultracentrifugation, anion echange chromatgraphy, and gel filtration	
1.1.2.7	native enzyme 22fold from strain AM1 in a single cation exchange chromatographical step, followed by ultrafiltration and buffer exchange, over 97% purity	
1.1.2.7	native enzyme 9fold to homogeneity by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography, followed by ultrafiltration and gel filtration	
1.1.2.7	native isozymes by anion exchange chromatography and gel filtration, type I 7.9fold, type II 14.7fold, the lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press	
1.1.2.7	recombinant selennomethionine-labeled, detagged MxaJ(residues 12-281) without signal peptide from Escherichia coli strain BL21(DE3) by anion-exchange chromatography and gel filtration	
1.1.2.7	soluble fraction concentrated with Centricon (Millipore, Billerica, Mass, USA), applied to a POROS 20 HQ column, followed by FPLC Superose 12 HR 10/30 column