1.1.1.45	-	
1.1.1.45	12%-52% yield	
1.1.1.45	67% yield	
1.1.1.45	ammonium sulfate fractionation, Sephadex G-100 column chromatography, Q-Sepharose column chromatography, Blue-Sepharose column chromatography, hydroxylapatite column chromatography	
1.1.1.45	recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) cells