1.1.1.316 - 1.1.1.316 ammonium sulfate precipitation, anion exchange chromatography, hydrophobic interaction chromatography and gel filtration, recombinant fusion protein by GST-affinity chromatography 1.1.1.316 ammonium sulfate precipitation, hydrophobic interaction chromatography, anion exchange chromatography, Blue Sepharose affinity chromatography and gel filtration 1.1.1.316 immobilized metal ion affinity chromatography (Ni2+) and gel filtration 1.1.1.316 Ni2+-charged HiTrap column chromatography and Superdex 200 pg gel filtration 1.1.1.316 partial