1.1.1.105 immobilized metal ion affinity chromatography 737923 1.1.1.105 Ni2+-NTA resin column chromatography 691750 1.1.1.105 preparation of RoDH-4 contains a minor contaminating protein band. Therefore, the exact concentration of RoDH-4 is determined based on the calibration curve constructed from several concentrations of BSA loaded on to the same SDS/PAGE gel 667537 1.1.1.105 RDH1 activity during purification is maintained only with phosphotidylcholine 721724 1.1.1.105 recombinant enzyme 691750 1.1.1.105 Sephacryl S300 gel filtration 740085 1.1.1.105 solubilized enzyme is extremely unstable and loses almost 80% of the initial activity even at partially purified stage, purification uses resistance of enzyme to proteolysis at 4°C in the presence of 1% Triton 654713