4.2.1.96	-	
4.2.1.96	at 4°C	
4.2.1.96	by affinity chromatography over a HisTrap nickel chelating column, followed by TEV cleavage/dialysis at 4°C overnight and then further passage over the a HisTrap nickel column to remove the tag, contaminants and the His-tagged TEV protease. A final purification step using a Superdex 75 size-exclusion column is performed	
4.2.1.96	DcoH2 (dimerization cofactor of HNF1alpha)	
4.2.1.96	recombinant	
4.2.1.96	recombinant enzyme	
4.2.1.96	recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, tag cleavage by thrombin, and p-aminobenzamidine affinity chromatography	
4.2.1.96	wild type and mutant enzymes Cys81Ser and Cys81Arg	
4.2.1.96	wild type and mutant enzymes E69A, D100N, H73A, H74A, H91A, W81A, and G72S and double-mutant H73A,H74A expressed in Escherichia coli