3.4.21.99 proteolytic modification preprospermosin has a MW of 41896 Da. The N-terminal sequence of the preprospermosin corresponds to a signal peptide for a nascent protein destined for initial transfer to the endoplasmic reticulum. The pro-form of spermosin may start from Ser23 651146 3.4.21.99 proteolytic modification spermosin is synthesized as a preproenzyme with a molecular mass of 42000 Da, consisting of 388 amino acid residues. The N-terminal 22 residues of preprospermosin correspond to a signal peptide. There are two forms of spermosin in the sperm: the 33000 Da spermosin is made up of the heavy chain (residues 130-388) and the light chain (residues 97-129). The 40000 Da enzyme form consists of the heavy chain (residues 130-388) and the light chain (residues 23-129) 665085