4.2.1.39 synthesis highly economic one-step biocatalytic synthesis procedure of 2-keto-3-deoxy-D-gluconate. D-Gluconate is completely converted tostereochemically pure 2-dehydro-3-deoxy-D-gluconate without side-product formation. The final 2-dehydro-3-deoxy-D-gluconate yield is approximately 90% -, 728022 4.2.1.39 synthesis highly economic one-step biocatalytic synthesis procedure of 2-oxo-3-deoxy-D-gluconate from D-gluconate using recombinant gluconate dehydratase (GAD) from the hyperthermophilic crenarchaeon Thermoproteus tenax -, 728022 4.2.1.39 synthesis one-step biocatalytic synthesis procedure for 2-keto-3-deoxy-D-gluconate from D-gluconate using recombinant gluconate dehydratase. The D-gluconate is completely converted to stereochemically pure D-2-keto-3-deoxy-D-gluconate without side-product formation, the final 2-keto-3-deoxy-D-gluconate yield is approximately 90%, the quantitative and qualitative LC-MS analysis method enables the simultaneous detection of D-gluconate and 2-keto-3-deoxy-D-gluconate and the enzyme can be provided by a simple and rapid procedure involving only two precipitation steps -, 748263 4.2.1.39 synthesis utilization for fermentation 2-keto-3-deoxy-D-gluconate proposed, conditions for fermentation 5687, 5688