1.1.1.274	biotechnology	enzyme is a target for the construction of a NADH-utilizing mutant strain in the industrial production of vitamin C	657308	
1.1.1.274	synthesis	enzymatic production of the vitamin C precursor 2-keto-L-gulonate (2-KLG) from 2,5-diketo-D-gluconate (2,5-DKG) by coupling 2,5-diketo-D-gluconic acid reductase via its coenzyme to glucose dehydrogenase. The bienzymatic process shows complicated inhibition patterns caused by reaction products, NADP+ and NADPH. The key parameters for a fast and efficient conversion are the NADP(H) concentration, the volumetric activity of 2,5-DKG reductase, the ratio of synthetic enzyme activity to regenerate enzyme activity and the glucono-1,5-lactone concentration. By modeling the space-time yield of the process is nearly doubled and the coenzyme concentration reduced threefold	740008	
1.1.1.274	synthesis	enzyme can be used in the industrial production of vitamin C	657308	
1.1.1.274	synthesis	enzyme may be useful for the synthesis of the ascorbate precursor 2-keto-L-gulonate	440306