2.6.1.11 biotechnology the pCR2.1-argD complementation plasmid is stably maintained in the argD(1000)::Tn5 transposon mutant growing in host tissues without any antibiotic selection. The pCR2.1-argD complementation plasmid can be useful for the expression of genes, markers, and reporters in Erwinia amylovora growing in planta, without concern about losing the plasmid over time -, 737502