5.4.99.15	synthesis	overexpression of the enzyme treY gene and the treY/treZ synthetic operon encoding enzyme and maltooligosyltrehalose trehalohydrolase significantly increases maltooligosyltrehalose synthase activity, the rate-limiting step, and improves the specific productivity and the final titer of trehalose. Furthermore, a strong decrease is noted in glycogen accumulation. Expression of UDP-glucose pyrophosphorylase/maltooligosyltrehalose synthase and UDP-glucose pyrophosphorylase/maltooligosyltrehalose synthase/maltooligosyltrehalose trehalohydrolase synthetic operons shows a partial recovery in the intracellular glycogen levels and a significant improvement in both intra- and extracellular trehalose content	
5.4.99.15	synthesis	production of trehalose by enzyme plus maltooligosyltrehalose trehalohydrolase is increased in presence of 4-alpha-glucanotransferase, converting 70% of maltopentaose into trehalose after 6 h, while 60% is converted without 4-alpha-glucanotransferase	
5.4.99.15	synthesis	conditions for the production of trehalose from starch by thermostable maltooligosyl trehalose synthase and maltooligosyl trehalose trehalohydrolase from Sulfolubus acidocaldarius ATCC 33909	
5.4.99.15	synthesis	trehalose production from starch