2.5.1.115 agriculture stress-induced expression of VTE2 can be used to increase the vitamin E content and to diminish detrimental effects of environmental stress in plants 2.5.1.115 agriculture the transient expression of the enzyme gene enhances the vitamin E levels in transgenic Nicotiana tabacum leaves. The stable expression of the enzyme gene is an efficient strategy to enhance vitamin E biosynthesis in agricultural crop breeding 2.5.1.115 nutrition in leaves, isoform HPT1 constitutive overexpression results in a 10fold increase in HPT specific activity and a 4.4fold increase in total tocopherol content relative to wild type. In seeds, HPT1 overexpression results in a 4fold increase in HPT specific activity and a total seed tocopherol content that is 40% higher than wild type, primarily because of an increase in gamma-tocopherol content. This enlarged pool of gamma-tocopherol is almost entirely converted to alpha-tocopherol by crossing HPT1 overexpressing plants with lines constitutively overexpressing gamma-tocopherol methyltransferase. Seed of the resulting double-overexpressing lines displays a 12fold increase in vitamin E activity relative to wild type 2.5.1.115 synthesis constitutive expression of genes encoding homogentisate phytyltransferase HPT and tocopherol cyclase TC individually and in combination in tobacco. The alpha-tocopherol content in transgenic tobacco plants expressing HPT, TC, and both HPT and TC is increased by 5.4-, 4.0-, and 7.1fold, respectively, when compared to the wild type