2.1.1.63 2,2,2-trifluoroethanol enzyme retains its native structure at high concentrations 659092 2.1.1.63 2-propanol enzyme retains its native structure at high concentrations 659092 2.1.1.63 Ethanol enzyme retains its native structure at high concentrations 659092 2.1.1.63 Methanol enzyme retains its native structure at high concentrations 659092 2.1.1.63 SDS enzyme retains its native structure at high concentrations 659092 2.1.1.63 urea 5 mM, melting temperature of wild-type enzyme: 91.5 °C, melting temperature of mutant enzyme E83A: 89.2°C, melting temperature of mutant enzyme E93A: 85.5°C, melting temperature of mutant enzyme E158A: 90.6°C, melting temperature of mutant enzyme E159A: 91.7 °C 659089