6.3.2.29	ATP + [L-Asp(4-L-Arg)]n + L-Asp	-	
6.3.2.29	ATP + [L-Asp(4-L-Arg)]n + L-Asp	study of expression profiles of the genes cphA1 and cphA2 and their dependence on the type of nitrogen supply in the medium	
6.3.2.29	additional information	without L-arginine 1.1% activity compared to the reaction mixture containing both substrates, no activity using L-lysine instead of L-arginine, no activity without addition of small amounts of cyanophycin as a primer for synthesis	
6.3.2.29	additional information	CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview	
6.3.2.29	additional information	cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers	
6.3.2.29	[L-Asp(4-L-Arg)]n + L-Asp + ATP	a small amount of cyanophycin is required as a primer