6.1.1.17 ATP + L-glutamate + tRNAGln - 6.1.1.17 ATP + L-glutamate + tRNAGln enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli 6.1.1.17 ATP + L-glutamate + tRNAGln isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli 6.1.1.17 ATP + L-glutamate + tRNAGln(CUG) the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) 6.1.1.17 ATP + L-glutamate + tRNAGln(UUG) the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) 6.1.1.17 ATP + L-glutamate + tRNAGlu - 6.1.1.17 ATP + L-glutamate + tRNAGlu isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli 6.1.1.17 ATP + L-glutamate + tRNAGlu recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli 6.1.1.17 ATP + L-glutamate + tRNAGlu eukaryotic-type discriminating glutamyl-tRNA synthetase, inability to utilize Escherichia coli tRNA as substrate. The enzyme is essential for growth of insect stage Trypanosoma brucei and is responsible for essentially all of the glutamyl-tRNA synthetase activity in cytosol and in mitochondria 6.1.1.17 ATP + L-glutamate + tRNAGlu glutamyl-tRNA, formed by Glu-tRNA synthetase, is a substrate for protein biosynthesis and tetrapyrrole formation by the C5 pathway