3.4.21.98	CARDIF + H2O	NS3-4A cleaves Cardif (adaptor protein of retinoic acid-inducible gene I or melanoma differentiation-associated gene-5), resulting in an interruption of the Cardif-mediated pathway in non-neoplastic human hepatocyte PH5CH8 cells	
3.4.21.98	CARDIF + H2O	NS3-4A cleaves Cardif (CARD adaptor-inducing) by generating a 70 kDa fragment. NS3-4A-mediated cleavage, is not caspase-dependent. Cardif mutant C508A is resistant to NS3-4A-mediated cleavage	
3.4.21.98	caspase recruitment domain adaptor-inducing IFN-beta (Cardif) + H2O	-	
3.4.21.98	hepatitis C virus polyprotein + H2O	mutational cleavage	
3.4.21.98	hepatitis C virus polyprotein + H2O	the enzyme is essential for processing of viral polyprotein	
3.4.21.98	hepatitis virus C polyprotein + H2O	maturation of the viral polyprotein	
3.4.21.98	mitochondrial antiviral signaling protein + H2O	i.e. MAVS, cleavage at Cys-508	
3.4.21.98	additional information	NS3-4A lacks the ability to cleave endogenous TRIF, regardless of HCV strains derived from patients with different stages of hepatic disease. Inflammatory cytokine production by NF-kappaB activation via the TRIF-mediated pathway also remains unsuppressed by NS3-4A	
3.4.21.98	additional information	NS3/4A protease is responsible for cleaving the viral polyprotein at junctions 3-4A, 4A4B, 4B5A, and 5A5B and two host cell adaptor proteins of the innate immune response, TRIF and MAVS