1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+ preferred substrate 1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+ Paenibacillus polymyxa ATCC 12321 has the ability to form almost exclusively the R isomer of 2,3-BDL (over 98%) when grown under anaerobic conditions 1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+ - 1.1.1.4 (2R,3S)-butane-2,3-diol + NAD+ - 1.1.1.4 (2S)-acetoin + NADH + H+ the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate 1.1.1.4 (2S,3S)-butane-2,3-diol + NAD+ - 1.1.1.4 (R)-acetoin + NADH + H+ - 1.1.1.4 (R)-acetoin + NADH + H+ the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 1.1.1.4 (R)-acetoin + NADH + H+ high level production of (R,R)-butane-2,3-diol 1.1.1.4 (R,R)-butane-2,3-diol + NAD+ -