1.14.13.25 formate + NAD(P)H + O2 assay with whole cells 1.14.13.25 methane + duroquinol + O2 - 1.14.13.25 methane + NAD(P)H + H+ + O2 - 1.14.13.25 methane + NAD(P)H + H+ + O2 methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system 1.14.13.25 methane + NAD(P)H + O2 - 1.14.13.25 methane + NAD(P)H + O2 initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source 1.14.13.25 methane + NADH + H+ + O2 - 1.14.13.25 methane + NADH + O2 - 1.14.13.25 methane + NADH + O2 methane is oxidized to methanol with 100% efficiency with no over-oxidation, methanol is then further oxidized by other enzymes in two electron steps to CO2 1.14.13.25 methane + NADH + O2 via diiron(IV) reaction intermediate Q, the decay rate of intermediate Q is substantially accelerated in the presence of fluuoromethane and difluoromethane