1.1.1.31	(S)-3-hydroxyisobutyrate + NAD+	-	
1.1.1.31	(S)-3-hydroxyisobutyrate + NAD+	the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor	
1.1.1.31	3-hydroxy-2-methylpropanoate + NAD+	-	
1.1.1.31	3-hydroxy-2-methylpropanoate + NADP+	-	
1.1.1.31	D,L-3-hydroxyisobutyrate + NAD+	-	
1.1.1.31	D,L-3-hydroxyisobutyrate + NAD+	strain IFO 1542	
1.1.1.31	D,L-3-hydroxyisobutyrate + NAD+	strain IFO 0750	
1.1.1.31	L-3-hydroxyisobutyrate + NAD+	-	
1.1.1.31	additional information	the enzyme catalyzes the rate-limiting step in the irreversible degradative pathway for the carbon skeleton of valine and the other branched-chain amino acids	
1.1.1.31	additional information	MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59