3.4.21.4	Gelatin + H2O	-	
3.4.21.4	protease-activated receptor-1 + H2O	activation	
3.4.21.4	protease-activated receptor-2 + H2O	activation	
3.4.21.4	protease-activated receptor-3 + H2O	activation	
3.4.21.4	protease-activated receptor-4 + H2O	activation	
3.4.21.4	additional information	active 1918 pandemic flu viral neuraminidase is resistant to trypsin digestion	
3.4.21.4	additional information	capable of agglutinating both bloodstream-form and procyclic trypanosomes as well as rabbit red blood cells	
3.4.21.4	intestinal alkaline sphingomyelinase bound to mucosa + H2O	cleavage at R440, first tryptic site upstream of signal anchor. Physiological role of enzyme in sphingomyelin digestion	
3.4.21.4	additional information	does not cleave enhanced green fluorescent protein-T1m and wild type enhanced green fluorescent protein	
3.4.21.4	enhanced green fluorescent protein-T1 + H2O	enhanced green fluorescent protein-T1 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 20 and 8 kDa	
3.4.21.4	enhanced green fluorescent protein-T2 + H2O	enhanced green fluorescent protein-T2 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 18 and 10 kDa	
3.4.21.4	additional information	enzyme pancreatic isoforms cationic trypsin and anionic trypsin show activity on human proteinase-activated receptors PAR-1, PAR-2, PAR-3 comparable to that of bovine pancreatic trypsin. Mesotrypsin cannot activate or disable proteinase-activated receptors in human epithelial cells, but activates PAR-1 in human astrocytoma cells	
3.4.21.4	additional information	salmon trypsin induces interleukin-8 production in human airway epithelial cells, purified salmon trypsin activates nuclear factor-kappa B-mediated gene expression in HeLa cells overexpressing human protease-activated receptor-2, the production of interleukin-8 by salmon trypsin is mediated by protease-activated receptor-2	
3.4.21.4	additional information	the sequence of trypsin is IVGGY	
3.4.21.4	additional information	trypsin is involved in the progesterone-induced acrosome reaction in canine spermatozoa	
3.4.21.4	DrmSP8-36 + H2O	trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph	
3.4.21.4	additional information	trypsin specifically cleaves the peptide bond on the carboxyl side of Lys and Arg residues. It is characterized by a common catalytic mechanism involving the catalytic triad of three essential amino acid residues His57, Asp102, and Ser195 and a substrate determinant residue Asp189	
3.4.21.4	additional information	trypsin treatment alters a number of characteristics of KATP channel pharmacology, this may be due to action at possibly more than one site but includes the functional cleavage of the sulfonylurea receptor from the KATP channel	
3.4.21.4	additional information	trypsin treatment increases zone of polarizing activity signaling of non-zone of polarizing activity tissue	
3.4.21.4	additional information	trypsin-catalyzed peptide synthesis with inverse substrates as acyl donor components, overview	
3.4.21.4	human cartilage collagen type II + H2O	use of enzyme and its regulators as markers of prognosis and disease activity in rheumatoid arthritis