3.1.21.7	deoxyinosine-containing deoxyoligonucleotide + H2O	the enzyme is involved in damaged DNA repair	
3.1.21.7	DNA + H2O	5'-GCTCGGCTICGGACCGAG-3'. Endonuclease V is an inosine-specific nucleases that cleave at the second phosphodiester bond 3' to inosine	
3.1.21.7	DNA + H2O	endonuclease V recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion in damaged DNA. The enzyme possesses specific endonuclease activity for the deoxyinosine-containing DNA strand	
3.1.21.7	DNA + H2O	the enzyme binds A:TT loops with higher affinity than undamaged DNA	
3.1.21.7	DNA + H2O	the enzyme cleaves the second phosphodiester bond 3' to deoxyinosine	
3.1.21.7	DNA + H2O	endonuclease V is an important enzyme for repairing deoxyinosine in DNA	
3.1.21.7	DNA + H2O	-	
3.1.21.7	DNA + H2O	cleavage of both denatured and native eukaryotic DNA	
3.1.21.7	DNA + H2O	pyrimidine dimer-specific	
3.1.21.7	DNA + H2O	also nicks duplex DNA exposed to OsO4, x-rays or acid, does not act upon undamaged DNA or irradiated single-stranded DNA	
3.1.21.7	DNA + H2O	two distinct activities: pyrimidine dimer glycosylase and apurinic-apyrimidinic endonuclease, beta-elimination of the 3'-phosphate of an abasic site	
3.1.21.7	DNA + H2O	cleaves deoxyinosine-containing DNA, urea residues-containing DNA, apurinic sites, base mismatches, insertion/deletion mismatches, flaps and pseudo-Y structures	
3.1.21.7	DNA + H2O	pyrimidine dimer-specific base excision repair pathway	
3.1.21.7	DNA + H2O	moving a DNA base extrahelical opposite the target site that induces a conformational change to facilitate catalysis	
3.1.21.7	DNA + H2O	active at apurinic/apyrimidinic sites, UV light induced damages, adducts of 7-bromomethylbenz(a)anthracene, uracil-containing duplex DNA	
3.1.21.7	double-stranded plasmid DNA + H2O	-	
3.1.21.7	double-stranded plasmid DNA + H2O	predominant release of a beta-elimination product rather than a hydrolytic product	
3.1.21.7	double-stranded plasmid DNA + H2O	removal of 3'deoxytermini	
3.1.21.7	double-stranded plasmid DNA + H2O	10fold lower activity than for single-stranded circular DNA, relaxed plasmid DNA, exposure to UV light or sodium bisulfite, to pH 5.2 or osmium tetroxide renders duplex DNA 7fold more active, best substrate is uracil containing DNA from Bacillus subtilis	
3.1.21.7	double-stranded plasmid DNA + H2O	supercoiled pBR322-DNA	
3.1.21.7	additional information	endonuclease V is an enzyme that initiates a conserved DNA repair pathway by making an endonucleolytic incision at the 3'-side 1 nt from a deaminated base lesion. But Tma endonuclease V also exhibits inosine-dependent 3'-exonuclease activity and non-specific 5'-exonuclease activity	
3.1.21.7	additional information	the enzyme shows affinity for but does not cleave branched DNA substrates like 5'-flap, 3'-flap, pseudo-Y, fork, 3-way junction, and Holliday junction	
3.1.21.7	nicked DNA + H2O	-	
3.1.21.7	RNA + H2O	the enzyme also cleaves inosine-containing RNA strands	
3.1.21.7	RNA-DNA hybrid + H2O	the enzyme specifically cleaves the RNA strand of RNA-DNA hybrids. The enzyme is specific for inosine in RNA	
3.1.21.7	RNA-DNA hybrid + H2O	the enzyme specifically cleaves the RNA strand of RNA-DNA hybrids. The enzyme is specific for inosine in RNA. The enzyme cleaves an RNA substrate containing inosine in a position corresponding to a biologically important site for deamination in the Gabra-3 transcript of the GABAA neurotransmitter. Further, the enzyme specifically incises transfer RNAs with inosine in the wobble position	
3.1.21.7	single-stranded circular DNA + H2O	10fold more activity than for duplex DNA	
3.1.21.7	single-stranded RNA + H2O	5'-CUGACUICGGAUCAGGGCC-3'. The enzyme is most active towards single-stranded RNA but is much less active towards other substrates	
3.1.21.7	single-stranded RNA + H2O	in prokaryotes, endonuclease V (EndoV) can recognize and cleave inosine-containing DNA. In contrast, mammalian EndoVs preferentially cleave inosine-containing RNA, suggesting a role in RNA metabolism for the eukaryotic members of this protein family