3.1.11.2 25500 26000 gel filtration 3.1.11.2 29000 - 2 * 29000, SDS-PAGE, wild-type and mutant enzymes E117Q and E117D 3.1.11.2 58000 - SDS-PAGE 3.1.11.2 95000 - determined by SDS-PAGE 3.1.11.2 100000 - 1 * 100000 3.1.11.2 100000 - 1 * 100000, likely it is cleaved by the action of endogenous proteases during the purification procedure. As a consequence of that, two proteolytic fragments of about 50 and 40 kDa, in addition to the intact 100-kDa molecular species, can be detected upon SDS-PAGE