2.5.1.1 38000 - x * 38000, SDS-PAGE 739121 2.5.1.1 39600 - - 738211 2.5.1.1 40000 - x * 40000, SDS-PAGE of recombinant protein 706332 2.5.1.1 45800 - 2 * 45800, the enzyme is always present as a dimer regardless of the added cofactor, in the case of Mg2+, the dimeric protein possesses the largest volume (93800 Da), most likely due to a more relaxed conformation. With Co2+, the enzyme seems to have a more compact conformation (87600 Da), which may be responsible for the change in product spectrum calculated from sequence 723664 2.5.1.1 49400 - - 739532 2.5.1.1 87600 - enzyme-Co2+ complex, gel filtration 723664 2.5.1.1 93800 - enzyme-Mg2+ complex, gel filtration 723664 2.5.1.1 158000 - gel filtration 738211