4.1.99.2	K+	5-10 mM, accelerates activity	33465	
4.1.99.2	K+	activating	678401	
4.1.99.2	K+	activation	651062	
4.1.99.2	K+	catalytically important	678198	
4.1.99.2	K+	K+ or NH4+ required for maximal activity	33459	
4.1.99.2	additional information	divalent cations (Mg2+, Ca2+, Ba2+, and Sr2+) do not activate TPL	678198	
4.1.99.2	additional information	the enzyme requires a monovalent cation, either K+, NH4+, Rb+ or Cs+ for activity, with Na+ and Li+ giving little or no activity. The cation is bound by the gamma-carboxylate of a conserved glutamate residue Glu69 of the TPL peptide backbone carbonyl oxygens, and waters, and is located about 10 A from cofactor pyridoxal 5'-phosphate	747245	
4.1.99.2	NH4+	5-10 mM, accelerates activity	33465	
4.1.99.2	NH4+	K+ or NH4+ required for maximal activity	33459