3.6.1.45 Co2+ absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective 209944 3.6.1.45 K+ absolute requirement for K+ 32880 3.6.1.45 Mg2+ - 696080, 698591 3.6.1.45 Mg2+ absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective 209944 3.6.1.45 Mg2+ absolutely required for optimal activity, not replacable by Mn2+ 696080 3.6.1.45 Mg2+ activity is dependent on the presence of Mg2+ 711078 3.6.1.45 Mg2+ divalent metal ion required, significantly lower Km in presence of Mn2+ as compared with Mg2+ 32880 3.6.1.45 Mn2+ absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective 209944 3.6.1.45 Mn2+ divalent metal ion required, significantly lower Km in presence of Mn2+ as compared with Mg2+ 32880