3.1.3.89 Co2+ 0.1 mM used in assay conditions 749824 3.1.3.89 Co2+ presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+. Apparent Kd value for Co2+ 0.8 mM 656266 3.1.3.89 Cu2+ presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+ 656266 3.1.3.89 Mg2+ 5 mM used in assay conditions 749824 3.1.3.89 Mn2+ activates 749824 3.1.3.89 Mn2+ presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+ 656266