3.1.3.23 Co2+ stimulates to a lesser degree than Mg2+ 94727 3.1.3.23 Fe2+ stimulates to a lesser degree than Mg2+ 94727 3.1.3.23 Mg2+ absolutely required for catalysis, optimal at 15 mM 714982 3.1.3.23 Mg2+ contains Mg2+ in the active site 730458 3.1.3.23 Mg2+ optimum activity in presence of 5 mM Mg2+ plus 1 mM Mn2+ 750977 3.1.3.23 Mg2+ optimum concentration 2.5 mM 749666 3.1.3.23 Mg2+ required for activity 730667 3.1.3.23 Mg2+ the enzyme is dependent on divalent cations, Mg2+ is most effective, half-maximal stimulation at 1 mM 94727 3.1.3.23 Mn2+ optimum activity in presence of 5 mM Mg2+ plus 1 mM Mn2+ 750977 3.1.3.23 Mn2+ stimulates to a lesser degree than Mg2+ 94727