2.5.1.1 Co2+ required for the production of geranyl diphosphate 738382 2.5.1.1 Co2+ with dimethylallyl diphosphate and isopentenyl diphosphate the enzyme is far more active with Co2+ as an additive than with any other tested metal ion. In the presence of Co2+ or Mn2+, with dimethylallyl diphosphate as a cosubstrate, the enzyme produces about 96% geranyl diphosphate and only 4% farnesyl diphosphate. In contrast, with Mg2+ as an additive, PcIDS1 produces 18% geranyl diphosphate and 82% farnesyl diphosphate 723664 2.5.1.1 Mg2+ required 739121 2.5.1.1 Mg2+ required for activity 738211 2.5.1.1 Mg2+ required, activity reaches its highest value at 10 mM MgCl2. At 5 or 15 mM MgCl2, activity is about 65% of maximum 706332 2.5.1.1 Mg2+ with dimethylallyl diphosphate and isopentenyl diphosphate the enzyme is far more active with Co2+ as an additive than with any other tested metal ion. In the presence of Co2+ or Mn2+, with dimethylallyl diphosphate as a cosubstrate, the enzyme produces about 96% geranyl diphosphate and only 4% farnesyl diphosphate. In contrast, with Mg2+ as an additive, PcIDS1 produces 18% geranyl diphosphate and 82% farnesyl diphosphate 723664 2.5.1.1 additional information the enzyme is inactive without adding divalent metal cations 723664