1.13.11.33 Ca2+ - 639258 1.13.11.33 Ca2+ 1 mM 672376 1.13.11.33 Ca2+ 50% of maximal activity at 0.1 mM Ca2+ in presence of 5 mM EGTA 639250 1.13.11.33 Ca2+ activates reaction with crude extract 639253 1.13.11.33 Ca2+ assay in presence of Ca2+ 702247 1.13.11.33 Ca2+ at 0.005-0.05 mM is required for efficient membrane binding 659378 1.13.11.33 Ca2+ Ca2+ binds to the PLAT domain of 15-LOX-2, resulting in a translocation from the cytosol to the membrane 741942 1.13.11.33 Ca2+ enhances activity, reversible by EGTA, inhibited by phosphatidyl serine and phosphatidyl choline 639241 1.13.11.33 Ca2+ for maximal intracellular activity, 12/15-lipoxygenases require a rise in cytosolic calcium concentration inducing a translocation of the enzyme from the cytosol to cellular membranes 639258 1.13.11.33 Ca2+ in the presence of Ca2+, with or without calcium ionophore, the majority of 15-LO-1 is found in the membrane fraction, although significant amounts are also detected in the supernatant fraction 703628